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Enzyme Immobilization on a Delignified Bamboo Scaffold as a Green Hierarchical Bioreactor

https://doi.org/10.1021/acssuschemeng.2c00346

Ren, Yihua ; Zhang, Libo ; Sun, Tao ; Yin, Yingwu ; Wang, Qian ( May 2022 , ACS Sustainable Chemistry & Engineering)

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Enhanced P450 fatty acid decarboxylase catalysis by glucose oxidase coupling and co-assembly for biofuel generation

https://doi.org/10.1016/j.biortech.2020.123538

Zhang, Libo ; Manley, Olivia M. ; Ma, Dumei ; Yin, Yingwu ; Makris, Thomas M. ; Wang, Qian ( September 2020 , Bioresource Technology)

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Using Small Molecules to Enhance P450 OleT Enzyme Activity in Situ

https://doi.org/10.1002/chem.202100680

Zhang, Libo ; Ma, Dumei ; Yin, Yingwu ; Wang, Qian ( May 2021 , Chemistry – A European Journal)

Abstract
Cytochrome P450 OleT is a fatty acid decarboxylase that catalyzes the production of olefins with biofuel and synthetic applications. However, the relatively sluggish catalytic efficiency of the enzyme limits its applications. Here, we report the application of a novel class of benzene containing small molecules to improve the OleT activity. The UV‐Vis spectroscopy study and molecular docking results confirmed the high proximity of the small molecules to the heme group of OleT. Up to 6‐fold increase of product yield has been achieved in the small molecule‐modulated enzymatic reactions. Our work thus sheds the light to the application of small molecules to increase the OleT catalytic efficiency, which could be potentially used for future olefin productions.

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